Education Profile

  • Postdoctoral Fellow, Salk Institute for Biological Sciences, 2009
  • Ph.D., University of Georgia, 2007
  • B.Sc., Peking (Beijing) University, 2001

Research Interests

​Professor Li‘s research attempts to understand the molecular basis of the regenerative mechanisms that maintain the proper form and function of the human body. His research programs build on a multidisciplinary platform that integrates stem cell models with genome engineering, functional genomics, bioengineering and chemical screening to gain a holistic understanding of regeneration in its broadest sense, while keeping a commitment to fulfilling the translational promise of stem cell research.

Selected Publications

  • Bi, C.; Wang, L.; Fan, Y.; Yuan, B.; Ramos-Mandujano, G.; Zhang, Y.; Alsolami, S.; Wang, J.; Shao, Y.; Reddy, P.; Zhang, P.-Y.; Huang, Y.; Yu, Y.*; Izpisua Belmonte, J.C.*; Li, M.*, Single-cell Individual Full-length mtDNA Sequencing Uncovers Unexpected Heteroplasmy Shifts in mtDNA Editing. Nucleic Acids Research, Mar 31, 2023.
  • Bi, C.; Wang, L.; Fan, Y.; Yuan, B.; Alsolami, S.; Zhang, Y.; Zhou, X.; Zhang, P.-Y.; Huang, Y.; Yu, Y.*; Izpisua Belmonte, J.C.*; Li, M.*, Quantitative Haplotype-resolved Analysis of mtDNA Heteroplasmy in Human Single Oocytes, Blastoids, and Pluripotent Stem Cells, Nucleic Acids Research, April 4, 2023.
  • Giorgetti, A.; Gu, Y.; Suzuki K.; Li, M.*, Editorial: Developmental Models 2.0. Frontiers in Cell and Developmental Biology, Oct 12, 2022
  • Yuan, B.; Zhou, X.; Suzuki K.; Ramos-Mandujano, G.; Wang, M.; Tehseen, M.; Cortes-Medina, L.; Moresco, J. J.; Dunn, S.; Hernandez-Benitez, R.; Hishida, T.; Kim, N.Y.; Andijani, M. M.; Bi, C.; Ku, M.; Takahashi, Y.; Xu, J.; Qiu, J.; Huang, L.; Benner, C.; Aizawa, E.; Qu, J.; Liu, G.H.; Li, Z.; Yi, F.; Ghosheh, Y.; Shao, C.; Shokhirev, M.; Comoli, P.; Frassoni, F.; Yates, R. J. III; Fu, XD; Rodríguez Esteban, C; Hamdan, S.; Izpisua Belmonte, J.C.*; Li M.*, Wiskott-Aldrich Syndrome Protein Forms Nuclear Condensates and Regulates Alternative Splicing. Nature Communications, Jun 25, 2022
  • Fan, Y.; Min, Z.; Alsolami, S. M.; Ma, Z.; Zhang, E.; Chen, W.; Zhong, K.; Pei, W.; Kang, X.; Zhang, P.; Wang, Y.; Zhang, Y.; Zhan, L.; Zhu, H.; An, C.; Li, R.; Qiao, J.; Tan, T.*;, Li, M.*; Yu, Y.*, Generation of human blastocyst-like structures from pluripotent stem cells. Cell Discovery 2021, 7:81. Co-corresponding author.
  • Klein, S. G.; Alsolami, S. M.; Steckbauer, A.; Arossa, S.; Parry, A. J.; Ramos Mandujano, G.; Alsayegh, K.; Izpisua Belmonte, J. C.*; Li, M.*; Duarte, C. M.*, A prevalent neglect of environmental control in mammalian cell culture calls for best practices. Nature Biomedical Engineering 2021, 1-6. Co-corresponding author.
  • Zhang, S. ; Yuan, B. ; Lam, J. H.; Zhou, J.; Zhou, X.; Ramos-Mandujano, G.; Tian, X.; Liu, Y.; Han, R.; Li, Y.; Gao, X.*; Li, M.*; Yang, M.*, Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis. Cell Discovery 2021, 7 (1), 1-15. Co-corresponding author.
  • C Bi, G Mandujano, Y Tian, S Hala, J Xu, S Mfarrej, Y Tian, C. Rodriguez Esteban, E Nunex Delicado, F Alofi, A Khogeer, A Hashem, N Almontashiri, A Pain, JC Izpisua Belmonte, M Li, Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing medRxiv, June 14, 2020, Published March 31, 2021,, Med
  • Bi, C., Wang, L., Yuan, B., Zhou X. Li, Y., Wang, S., Pang, Y., Gao X., Huang Y., Li, M. Long-read individual-molecule sequencing reveals CRISPR-induced genetic heterogeneity in human ESCs. Genome Biol 21, 213 (2020).
  • G Mandujano and M Li, MARVICS: A Robust and Safe Magnetic Nanoparticle based RNA Extraction Method Compatible with Phenol-chloroform Inactivated Infectious Samples,, July 15, 2020,
  • Li M* and Izpisua Belmonte JC*. Organoids - Preclinical Models of Human Disease. New England Journal of Medicine. Feb 7, 2019. *co-corresponding author
  • Li Y, Han R, Bi C, Li M, Wang S, Gao X, DeepSimulator: a deep simulator for Nanopore sequencing, Bioinformatics, April 2, 2018, in press.
  • Li M* and Izpisua Belmonte JC*. Deconstruct pluripotency gene regulatory network. Nature Cell Biology. VOL 20, APRIL 2018, 382–392., Co-corresponding author
  • Alsaiari SK, Patil S, Alyami M, Alamoudi KO, Aleisa FA, Merzaban JS, Li M, and Khashab NM, Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework. Journal of the American Chemical Society 2018 140 (1), pp 143–146 epub December 22, 2017
  • Takahashi Y, Wu J, Suzuki K, Martinez-Redondo P, Li M, Liao HK, Wu MZ, Hernandez-Benitez R, Hishida T, Shokhirev MN, Rodriguez Esteban C, Sancho-Martinez I, and Izpisua Belmonte JC. Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells. Science 356, 503–508 (2017) 5 May 2017 (link:
  • Li M and Izpisua Belmonte JC. Ground rules of the pluripotency gene regulatory network. Nature Reviews Genetics. Published online 03 January 2017
  • Li M and Izpisua Belmonte JC. Looking to the future following ten years of induced pluripotent stem cell (iPSC) technologies. Nature Protocols. 11, 1579-1585 (2016).
  • Ocampo A, Reddy P, Martinez-Redondo P, Platero-Luengo A, Hatanaka F, Hishida T, Li M, Lam D, Kurita M, Beyret E, Araoka T, Vazquez-Ferrer E, Donoso D, Roman JL, Xu J, Rodriguez Esteban D, Nunez G, Nunez Delicado E, Campistol JM, Guillen E, Guillen P, and Izpisua Belmonte JC. In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming. Cell 2016 Dec 15, 167, 1719-1733
  • Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim E, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Skowronska-Krawczyk D, Rodríguez Esteban C, Lajara J, Nuñez E, Guillen P, Campistol J, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway E, Zhang K, Izpisua Belmonte JC. In vivo genome editing via CRISPR-Cas9 mediated homology-independent targeted integration Nature 540, 144–149 (01 December 2016)