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Alfredo De Biasio

Assistant Professor, Bioscience

Biological and Environmental Science and Engineering Division


Affiliations

Education Profile

  • ​Lecturer, Leicester Institute of Structural and Chemical Biology, University of Leicester, UK, 2017
  • AIRC-Marie Curie Fellow, Elettra-Sincrotrone Trieste, Italy, 2014
  • Juan de la Cierva Postdoctoral Fellow, CIC bioGUNE, Bilbao, Spain 2009
  • PhD, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy 2008
  • M.Sc. in Chemistry, University of Padova, Italy 2001

Research Interests

​Dr. De Biasio's research interests revolve on the structural biology of human DNA replication and repair. His work makes use of cryogenic Electron Microscopy (cryo-EM) to obtain near-atomic resolution information on key enzymes operating in the human replisome, such as DNA polymerases. This structural data is combined with biochemical assays for a thorough understanding of the involved biomolecular processes.

Selected Publications

  • ​Lancey C, Tehseen M, Bakshi S, Percival M, Takahashi M, Sobhy MA, Raducanu VS, Blair K, Muskett FW, Ragan TJ, Crehuet R, Hamdan SM, De Biasio A.* Cryo-EM structure of human Pol κ bound to DNA and mono-ubiquitylated PCNA. Nat Commun. 2021 Oct 19;12(1):6095
  • Sobhy MA, Tehseen M, Takahashi M, Bralić A, De Biasio A, Hamdan SM. Implementing fluorescence enhancement, quenching, and FRET for investigating flap endonuclease 1 enzymatic reaction at the single-molecule level. Comput Struct Biotechnol J. 2021 Jul 27;19:4456-4471
  • Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A.* Structure of the processive human Pol δ holoenzyme. Nat Commun. 2020 Feb 28;11(1):1109
  • González-Magaña A, de Opakua AI, Merino N, Monteiro H, Diercks T, Murciano-Calles J, Luque I, Bernadó P, Cordeiro TN, Biasio A, Blanco FJ. Double Monoubiquitination Modifies the Molecular Recognition Properties of p15(PAF) Promoting Binding to the Reader Module of Dnmt1. ACS Chem Biol. 2019 Oct 18;14(10):2315-2326
  • De March M, Barrera-Vilarmau S, Crespan E, Mentegari E, Merino N, Gonzalez-Magaña A, Romano-Moreno M, Maga G, Crehuet R, Onesti S, Blanco FJ, De Biasio A.* p15(PAF) binding to PCNA modulates the DNA sliding surface. Nucleic Acids Res. 2018 Oct 12;46(18):9816-9828
  • De March M, De Biasio A*. The dark side of the ring: role of the DNA sliding surface of PCNA. Crit Rev Biochem Mol Biol. 2017 Dec;52(6):663-673
  • Cordeiro TN, Chen PC, De Biasio A, Sibille N, Blanco FJ, Hub JS, Crehuet R, Bernadó P. Disentangling polydispersity in the PCNA-p15PAF complex, a disordered, transient and multivalent macromolecular assembly. Nucleic Acids Res. 2017 Feb 17;45(3):1501-1515
  • De March M, Merino N, Barrera-Vilarmau S, Crehuet R, Onesti S, Blanco FJ, De Biasio A*. Structural basis of human PCNA sliding on DNA. Nat Commun. 2017 Jan10;8:13935
  • De Biasio A*, de Opakua AI, Mortuza GB, Molina R, Cordeiro TN, Castillo F, Villate M, Merino N, Delgado S, Gil-Cartón D, Luque I, Diercks T, Bernadó P, Montoya G, Blanco FJ. Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair. Nat Commun. 2015 Mar 12;6:6439
  • Lee CJ, De Biasio A, Beglova N. Mode of interaction between beta2GPI and lipoprotein receptors suggests mutually exclusive binding of beta2GPI to the receptors and anionic phospholipids. Structure. 2010 Mar 10;18(3):366-76